| 名稱 | c-Kit W557_K558del-D816V/BaF3 |
| 型號 | CBP73285 |
| 報價 |  |
| 特點(diǎn) | c-Kit W557_K558del-D816V/BaF3,母細(xì)胞：BaF3，KIT W557_K558del 導(dǎo)致 Kit 蛋白的近膜結(jié)構(gòu)域（外顯子 11）中從氨基酸 557 到 558（PMID：12918066）的 2 個氨基酸缺失。 W557_K558del 導(dǎo)致 Kit 的組成型激活和 Erk 磷酸化增加，并導(dǎo)致細(xì)胞培養(yǎng)中 ETV1 的侵襲性和表達(dá)增加。 |
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藥靶細(xì)胞株 > kinase激酶細(xì)胞株 > CBP73285c-Kit W557_K558del-D816V/BaF3
- 詳細(xì)內(nèi)容
CBP73285 | |
| I. Introduction | |
Cell Line Name： | c-Kit W557_K558del-D816V/BaF3 |
Host Cell: | Ba/F3 |
| Stability: | 16 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
Application: | Anti-proliferation assay and PD assay |
Freeze Medium: | 90% FBS+10% DMSO |
Complete Culture Medium: | RPMI-1640+10%FBS |
Mycoplasma Status: | Negative |
| II.Background | |
KIT W557_K558del results in the deletion of 2 amino acids in the juxtamembrane domain (exon 11) of the Kit protein from amino acids 557 to 558 (PMID: 12918066). W557_K558del results in constitutive activation of Kit and increased Erk phosphorylation, and leads to increased invasiveness and expression of ETV1 in cell culture (PMID: 25239608, PMID: 26936919). | |
| III. Representative Data | |
1. WB of c-Kit W557_K558del-D816V/BaF3
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2. Sanger of c-Kit W557_K558del-D816V/BaF3
Figure 2. c-Kit W557_K558del-T670I/BaF3 D816V
Figure 3. c-Kit W557_K558del-T670I/BaF3 W557_K558del | |
3. Anti-proliferation assay
Figure 4. CTG Proliferation Assay of BaF3 c-KIT W557_ K558del/D816V (C5). | |
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