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藥靶細胞株 > 腫瘤免疫細胞株 > CBP74009CD40/NFkB-Luc/HEK293

名稱	CD40/NFkB-Luc/HEK293
型號	CBP74009
報價
特點	CD40/NFkB-Luc/HEK293，凍存條件：90% FBS+10% DMSO

詳細內(nèi)容

I. Background
CD40 是腫瘤壞死因子受體超家族（TNFRSF）成員，作為免疫調(diào)節(jié)的關鍵分子，廣泛參與 B 細胞活化、抗原呈遞細胞（APC）成熟及 T 細胞免疫應答。其配體 CD40L（CD154）主要表達于活化的 T 細胞，通過 CD40-CD40L 互作橋接先天性與適應性免疫，是治療、自身免疫病及感染性疾病的熱門靶點。 CD40 信號轉(zhuǎn)導主要利用 TNF 受體相關因子的銜接蛋白，導致經(jīng)典和非經(jīng)典 NFκB 通路、MAPK、PI3K 和 PLCγ的激活。它們的激活導致特征性下游效應，包括轉(zhuǎn)錄激活、細胞骨架重排和細胞存活。

II. Description
CD40 NFκB-Luc HEK293 報告基因藥靶模型很好的模擬了體內(nèi) CD40 的信號轉(zhuǎn)導過程，原理見下圖所示。 Figure 1. CD40 NFκB-Luc HEK293 細胞模型原理圖

III. Introduction
Host Cell:	HEK293
Expressed gene:	CD40
Stability:	32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.)
Synonym(s):	NF-κB cell line, NFkappaB cell line, luciferase reporter, NF-κB cell line, NFκB cell line, CD40 cell line, cd40 luciferase reporter, CD40 HEK293 cell line
Freeze Medium:	90% FBS+10% DMSO
Culture Medium:	MEM+10%FBS+1% NEAA+1 mM NaP+1 μg/ml Puromycin+100 μg/ml Hygromycin B
Mycoplasma Testing:	Negative
Storage:	Liquid nitrogen
Application(s):	Functional(Report Gene) Assay

IV. Description of Host Cell Line
Organism:	Human
Tissue:	kidney
Morphology:	Epitheloid cell
Growth Properties:	Adherent

Ⅴ. Representative Data
Figure 2. Recombinant HEK293 cell line expressing firefly luciferase gene under the control of NFκB response elements with constitutive expression of CD40.
Figure 3. Dose Response of Human CD40L in CD40 NFκB-Luc HEK293( C4). Figure 4. Detect Luciferase assay by Ultra Luciferase Detection Kit CBPH0001（we strongly suggest to purchase from Cobioer). Blocking of hCD40L induced Activity by CD40L Blocking Ab in CD40 NFκB-Luc HEK293(C4). Blocking of CD40L induced Activity by CD40L Blocking Ab in CD40 NFκB-Luc HEK293 Cell(C4) with CD40L Target Cell(C7).